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Principales publications associées au PIQ


P. DIDIER, J. GODET, & Y. MELY. Two-photon two-focus fluorescence correlation spectroscopy (2fFCS) with a tunable distance between the excitation volumes. J. Fluorescence, 2009, 19, 561-565.

A.S. KLYMCHENKO, S. ONCUL, P. DIDIER, E. SCHAUB, L. BAGATOLLI, G. DUPORTAIL, & Y. MELY. Visualization of lipid domains in giant unilamellar vesicles using an environment-sensitive membrane probe based on 3-hydroxyflavone. Biochem. Biophys. Acta, Biomembranes, 2009, 1788, 495-499.

B. ILIEN, N. GLASSER, J.P. CLAMME, P. DIDIER, E. PIEMONT, R. CHINNAPAN, S. DAVAL, J.L. GALZI, & Y. MELY. Pirenzepine promotes the dimerization of muscarinic M1 receptors through a three-step binding process. J Biol Chem, 2009, 284, 19533-19543.

P. DIDIER, G ULRICH, Y. MELY, & R. ZIESSEL. Improved Push-Pull-Push E-Bodipy Fluorophores for Two-photon Cell-Imaging. Org. Biomol. Chem., 2009, 7, 3639-3942.

A. HAYEK, F. BOLZE, C. BOURGOGNE, P.L. BALDECK, P. DIDIER, Y. ARNTZ, Y. MÉLY, & J.F. NICOUD. Boron containing two-photon absorbing chromophores. 2. Fine tuning of the one- and two-photon photophysical properties of pyrazabole based fluorescent bio-probes. Inorg. Chem., 2009, 48, 9112-9119.

A. HAMADI, T.B. DERAMAUDT, K. TAKEDA & P. RONDE. Src activation and translocation from focal adhesions to membrane ruffles contribute to formation of new adhesion sites. Cell. Mol. Life Sci. 2009, 66, 324-338.


J.V. FRITZ, D. DUJARDIN, P. DIDIER, J. DE MEY, J. L. DARLIX, Y. MELY, & H. DE ROCQUIGNY. HIV-1 Vpr oligomerization but not that of Gag directs the interaction between Vpr and Gag. J. Virol., 2010, 84, 1585-1596.

E. BOUTANT, P. DIDIER, A. NIEHL, Y. MELY, C. RITZENTHALER, & M. HEINLEIN. Fluorescent protein recruitment for demonstration and analysis of in vivo protein interactions. Plant J., 2010, 62, 171-177.

O.A. KUCHERAK, P. DIDIER, Y. MELY, & A.S. KLYMCHENKO. Fluorene Analogues of Prodan with Superior Fluorescence Brightness and Solvatochromism. J Phys Chem Lett, 2010, 1 , 616-620.

A. KULAKOWSKA, P. JURKIEWICZ, J. SYKORA, A. BENDA, Y. MELY, & M. HOF. Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers. J. Fluorescence, 2010, 20, 563-9.

S. ONCUL, A.S. KLYMCHENKO, O.A. KUCHERAK, A.P. DEMCHENKO, S. MARTIN, M. DONTENWILL, Y. ARNTZ, P. DIDIER, G. DUPORTAIL & Y. MELY. Liquid ordered phase in cell membranes evidenced by a hydration-sensitive probe: effects of cholesterol depletion and apoptosis. Biochim. Biophys. Acta – Biomembranes 2010; 1798, 1436-1443.

O.A. KUCHERAK, S. ONCUL, D. ZEINAB, D.A. YUSHCHENKO, Y. ARNTZ, P.DIDIER, Y. MELY, & A.S. KLYMCHENKO. Switchable Nile Red-based probe for cholesterol and lipid order at the outer leaflet of biomembranes. J Am. Chem. Soc., 2010, 132, 4907-16.

L. CHOULIER, V.V. SHVADCHAK, A. NAIDOOA, A.S. KLYMCHENKO, Y. MELY, & D. ALTSCHUH. A peptide-based fluorescent ratiometric sensor for quantitative detection of proteins. Anal Biochem., 2010, 401, 188-195.

V.J. GREINER, C. EGELE, S. ONCUL, F. RONZON, C. MANIN, A. KLYMCHENKO, & Y. MELY. Characterization of the lipid and protein organization in HBsAg viral particles by steady-state and time resolved fluorescence spectroscopy. Biochimie, 2010, 92, 994-1002.

K. AMARI, E. BOUTANT, C. HOFMANN, C. SCHMITT-KEICHINGER, L. FERNANDEZ-CALVINO, P. DIDIER, A. LERICH, J. MUTTERER, C. THOMAS, Y. MELY, A. MAULE, & C. RITZENTHALER. Plasmodesmal proteins with receptor-like properties for plant viral movement proteins. Plos Pathogens. 2010., 6, e1001119.

N. JAIN, Y. ARNTZ, V. GOLDSCHMIDT, G. DUPORTAIL, Y. MELY, & A.S. KLYMCHENKO. New unsymmetrical bolaamphiphiles: synthesis, assembly with DNA and application for gene delivery. Bioconjug Chem. 2010, 21, 2110-2118.

A. HAMADI, T.B. DERAMAUDT, K. TAKEDA & P. RONDE. Hyperphosphorylated FAK delocalizes from focal adhesions to membrane ruffles. J. Oncol. 2010, Article ID 932803. E. MARTINKOVA, A. MAGLOTT, D.Y. LEGER, D. BONNET, M. STIBOROVA, K. TAKEDA, S. MARTIN & M. DONTENWILL. Alpha5beta1 integrin antagonists reduce chemotherapy-induced premature senescence and facilitate apoptosis in human glioblastoma cells. Int. J. Cancer 2010, 127, 1240-1248.


V. Y. POSTUPALENKO, V. V. SHVADCHAK, G. DUPORTAIL, V. G. PIVOVARENKO, A. S. KLYMCHENKO, & Y. MELY. Monitoring membrane binding and insertion of peptides by two-color fluorescent label. Biochim Biophys Acta- Biomembranes, 2011, 1808, 424-32.

A. PERRINO, A. KLYMCHENKO, A. MORERE, E CONTAL, A RAMEAU, J.M. GUENET, Y MELY, & A. WAGNER. Insight into the structure and behaviour of polydiacetylene-based micelles. Macromol. Chem. Phys., 2011, 212, 111-117.

E. CONTAL, A. KLYMCHENKO, Y. MELY, S. MEUNIER, & A. WAGNER. Core functionalization of polydiacetylene micelles by « click » reaction. Soft matter, 2011, 7, 1648-1650.

N. ROCHEL, F. CIESIELSKI, J. GODET, E. MOMAN, M. ROESSLE, C. PELUSO-ILTIS, M. MOULIN, M. HAERTLEIN, P. CALLOW, Y. MELY, D.I. SVERGUN & D. MORAS. Common architecture of nuclear receptor heterodimers on DNA direct repeat elements with different spacings. Nat. Struct. Mol. Biol. 2011, 18, 564-570.

R. V. RODIK, A.S. KLYMCHENKO, N. JAIN, S.I. MIROSHNICHENKO, L. RICHERT, V.I. KALCHENKO, & Y. MELY. Virus-sized DNA nanoparticles for gene delivery based on micelles of cationic calixarenes. Chem. Eur. J. 2011, 17, 5526-5538.

V. GREINER, V. SHVADCHAK, Y. ARNTZ, J FRITZ, P. DIDIER, B. FRISCH, C. BOUDIER, Y. MELY, & H. DE ROCQUIGNY. Characterization of the mechanisms of Vpr(52-96) internalization in cells. Biochimie, 2011, 93, 1647-58.

T.B. DERAMAUDT, D. DUJARDIN, A. HAMADI, F. NOULET, K. KOLLI, J. de MEY, K. TAKEDA & P. RONDE. FAK phosphorylation at Tyr-925 regulates cross-talk between focal adhesion turnover and cell protrusion. Mol. Biol. Cell 2011, 22, 964-975.

S. LECAT, M. OUEDRAOGO, T. CHERRIER, F. NOULET, P. RONDE, N. GLASSER, J.L. GALZI, Y. MELY, K. TAKEDA & B. BUCHER. Contribution of a tyrosine-based motif to cellular trafficking of wild-type and truncated NPY Y1 receptors. Cell. Signal. 2011, 23, 228-238.

E. SICK, A. BOUKHARI, T. DERAMAUDT, P. RONDE, B. BUCHER, P. ANDRE, J.P. GIES & K. TAKEDA. Activation of CD47 receptors causes proliferation of human astrocytoma but not normal astrocytes via an Akt-dependent pathway. Glia 2011, 59, 308-319.


S.V AVILOV, C. BOUDIER, M. GOTTIKH, J.L. DARLIX, & Y. MELY. Characterization of the inhibition mechanism of the HIV-1 nucleocapsid protein chaperone activities by methylated oligoribonucleotides. Antimicrob. Agents and Chemother. 2012, 56, 1010-1018.

K.K. SHARMA, H. DE ROCQUIGNY, J.L. DARLIX, J.P. LAVERGNE, F. PENIN, J.M. LESSINGER, & Y. MELY. Analysis of the RNA chaperoning activity of the Hepatitis C virus Core protein on the conserved 3’X region of the viral genome. Nucleic Acids Res., 2012, 40, 2540-2553.

A. JOUANONG, P. DIDIER, & Y. MELY. Identification of a thermally activated process in the Cy3 photobleaching mechanism. Phys. Chem. Chem. Phys. 2012, 14,1585-1588.

O. A. KUCHERAK, L. RICHERT, Y. MELY, & A.S. KLYMCHENKO. Dipolar 3-methoxychromones as highly fluorescent and photostable solvatochromic dyes. Phys. Chem. Chem. Phys. 2012, 14, 2292-2300.

N. JAIN, V. GOLDSCHMIDT, S. ONCUL, Y. ARNTZ, G. DUPORTAIL, Y. MELY, & A.S. KLYMCHENKO. Lactose-ornithine bolaamphiphiles for efficient gene delivery in vitro. Int. J. Pharm., 2012, 423, 392-400.

G. LERICHE, G. BUDIN, Z. DARWICH, D. WELTIN, Y. MELY, A. KLYMCHENKO, & A. WAGNER. A FRET-based probe with a chemically deactivatable quencher. Chem Comm. 2012, 48, 3224-3226.

V.J. GREINER, F. RONZON, E. LARQUET, B. DESBAT, C. ESTEVES, J. BONVIN, F. GRECO, C. MANIN, A. KLYMCHENKO, & Y. MELY. The structure of HBsAg particles is not modified upon their adsorption on aluminium hydroxide gel. Vaccine, 2012, 30, 5240-5245.

Z. DARWICH, A.S. KLYMCHENKO, O.A. KUCHERAK, L. RICHERT, & Y. MELY. Detection of apoptosis through the lipid order of the outer plasma membrane leaflet. Biochim. Biophys. Acta Biomembranes, 2012, 1818, 3048-3054.

A. NIEHL, K. AMARI, D. GEREIGE, K. BRANDNER, Y. MELY, & M. HEINLEIN. Control of Tobacco Mosaic Virus Movement Protein Fate by cell-division-cycle Protein 48 (CDC48). Plant Physiol., 2012, 24, 4465-4482.

A.S. KLYMCHENKO, E. ROGER, N. ANTON, H. ANTON, I. SHULOV, J. VERMOT, Y. MELY*, T. VANDAMME*. Highly lipophilic fluorescent dyes in nano-emulsions: towards bright non-leaking nano-droplets. RSC Adv., 2012, 2, 11876-11886.

J. BELLIS, I. DULUC, B. ROMAGNOLO, C. PERRET, M.C. FAUX, D. DUJARDIN, C. FORMSTONE, S. LIGHTOWLER, R.G. RAMSAY, J.N. FREUND & J.R. DE MEY. The tumor suppressor gene APC controls planar cell polarities central to gut homeostasis. J. Cell. Biol. 2012, 198, 331-341.

J. BRANTS, K. SEMENCHENKO, C. WASYLYK, A. ROBERT, A. CARLES, A. ZAMBRANO, K. PRADEAU-AUBRETON, C. BIRCK, J.A. SCHALKEN, O. POCH, J. DE MEY & B. WASYLYK. Tubulin tyrosine ligase like 12, a TTLL family member with SET- and TTL-Like domains and roles in histone and tubulin modifications and mitosis. PLoS ONE 7, e51258, 2012

E.C. COSSET, J. GODET, N. ENTZ-WERLE, E. GUERIN, D. GUENOT, S. FROELICH, D. BONNET, S. PINEL, F. PLENAT, P. CHASTAGNER, M. DONTENWILL & S. MARTIN. Involvement of the TGFβ pathway in the regulation of α5β1 integrins by caveolin-1 in human glioblastoma. Int. J. Cancer 2012, 131, 601-611.

G. CREUSAT, A. MAGLOTT-ROTH, B. PONS, M. DONTENWILL, E. GUERIN, J.S. THOMANN, B. FRISCH & G. ZUBER. Pyridylthiourea-grafted polyethylenimine offers an effective assistance to siRNA-mediated gene silencing in vitro and in vivo. J. Control. Release 2012, 157, 418-426.

H. JANOUSKOVA, A. MAGLOTT, D.Y. LEGER, C. BOSSERT, F. NOULET, E. GUERIN, D. GUENOT, S. PINEL, P. CHASTAGNER, F. PLENAT, N. ENTZ-WERLE, J. LEHMANN-CHE, J. GODET, S. MARTIN, J. TEISINGER & M. DONTENWILL. Integrin α5β1 plays a critical role in resistance to temozolomide by interfering with the p53 pathway in high grade glioma. Cancer Res. 2012, 72, 3463-3470.

K. KOLLI-BOUHAFS, A. BOUKHARI, A. ABUSNINA, E. VELOT, J.P. GIES, C. LUGNIER & P. RONDÉ. Thymoquinone reduces migration and invasion of human glioblastoma cells associated with FAK, MMP-2 and MMP-9 down-regulation. Invest. New Drugs 2012, 30, 2121-2131.

T. VERCRUYSSE, B. BASTA, W. DEHAEN, N. HUMBERT, J. BALZARINI, F. DEBAENE, S. SANGLIER-CIANFERANI, C. PANNECOUQUE, Y. MELY*, & D. DAELEMANS*. * co-last authors. A phenyl-thiadiazolylidene-amine derivative ejects zinc from retroviral nucleocapsid zinc fingers and inactivates HIV virions. Retrovirology. 2012, 9, 95.

JE. BASSARD, L. RICHERT, J. GEERINCK, H. RENAULT, F. DUVAL, P. ULLMANN, M. SCHMITT, E. MEYER, J. MUTTERER, W. BOERJAN, G. DE JAEGER, Y. MELY, A. GOOSSENS, D. WERCK-REICHHART. Protein-Protein and Protein-Membrane Associations in the Lignin Pathway. Plant Cell, 2012, 24, 4465-82.


A.S. RINALDI, G. FREUND, D. DESPLANCQ, A.P. SIBLER, M. BALTZINGER, N. ROCHEL, Y. MELY, P. DIDIER, & E. WEISS. The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells. Exp Cell Res. 2013. 319, 838-849.

J. GODET, C. KENFACK, F. PRZYBILLA, L. RICHERT, G. DUPORTAIL, & Y. MELY. Site-selective probing of cTAR destabilization highlights the necessary plasticity of the HIV-1 nucleocapsid protein to chaperone the first strand transfer. Nucleic Acids Res., 2013, 41, 5036-48.

B. MAILLOT, S. EILER, C. CRUCIFIX, F. GRANGER, L. RICHERT, P. DIDIER, J. GODET, N. LEVY, K. PRADEAU, S. EMILIANI, A NAZABAL, Y. MELY, D. MORAS, P. SCHULTZ, & M. RUFF. Structural and functional role of INI1 in the early step of HIV-1 infection. Plos One, 2013, 8, e60734.

Z. DARWICH, O.A. KUCHERAK, R. KREDER, L. RICHERT, R. VAUCHELLES, Y. MELY, A.S. KLYMCHENKO. Rational design of fluorescent membrane probes for apoptosis based on 3-hydroxyflavone. Methods Appl. Fluoresc., 2013, 1, 025002.

J. BATISSE, S.X. GUERRERO, S. BERNACCHI, L. RICHERT, J. GODET, V. GOLDSCHMIDT, Y. MELY, R. MARQUET, H. de ROCQUIGNY, & J.C. PAILLART. APOBEC3G impairs the multimerization of the HIV-1 Vif protein in living cells. J. Virol. , 2013, 87, 6492-506.

M. KRIFA, M. ALHOSIN, C.D. MULLER, J.P. GIES, L. CHEKIR-GHEDIRA, K. GHEDIRA, Y. MELY, C. BRONNER, & M. MOUSLI. Limoniastrum guyonianum aqueous gall extract induces apoptosis inhuman cervical cancer cells involving p16INK4A re-expression related to UHRF1 and DNMT1 down-regulation. J. Exp. Clin. Cancer Res., 2013, 32, 30.

M.F. PRODANOV, N.V. POGORELOVA, A.P. KRYSHTAL, A.S. KLYMCHENKO, Y. MELY, V.P. SEMYNOZHENKO, A.I. KRIVOSHEY, Y.A. REZNIKOV, S. YARMOLENKO, J.W. GOODBY, & V.V. VASHCHENKO. Thermodynamically Stable Dispersions of Quantum Dots in a Nematic Liquid Crystal. Langmuir, 2013, 29, 9301-9.

R. DAS, G. DUPORTAIL, A. GHOSE, L. RICHERT, A. KLYMCHENKO, S. CHAKRABORTYC, S. YESYLEVSKYY, & Y. MELY. Tuning Excited-State Proton Transfer Dynamics of a 3-hydroxychromone Dye in Supramolecular Complexes via Host-Guest Steric Compatibility. Phys. Chem. Chem. Phys. 2013, 16, 776-784.

H. JANOUSKOVA, A.M. RAY, F. NOULET, I. LELONG-REBEL, L. CHOULIER, F. SCHAFFNER, M. LEHMANN, S. MARTIN, J. TEISINGER & M. DONTENWILL. Activation of p53 pathway by Nutlin-3a inhibits the expression of the therapeutic target α5 integrin in colon cancer cells. Cancer Lett. 2013, 336, 307-318.

M. KRIFA, M. ALHOSIN, C.D. MULLER, J.P. GIES, L. CHEKIR-GHEDIRA, K. GHEDIRA, Y. MELY, C. BRONNER & M. MOUSLI. Limoniastrum guyonianum aqueous gall extract induces apoptosis inhuman cervical cancer cells involving p16INK4A re-expression related to UHRF1 and DNMT1 down-regulation. J. Exp. Clin. Cancer Res., 32, 30, 2013

F SCHAFFNER, A.M. RAY & M. DONTENWILL. Integrin α5β1, the fibronectin receptor, as a pertinent therapeutic target in solid tumors. Cancers 2013, 5, 27-47.


A. FERRAND, S. EAP, L. RICHERT, S. LEMOINE, D. KALASKAR, S. DEMOUSTIER-CHAMPAGNE, H. ATMANIF, Y. MELY, D. MAINARD, G. SCHLATTER, L. KUHN, G. LADAM, N. BENKIRANE-JESSEL. Osteogenetic Properties of Electrospun Nanofibrous PCL Scaffolds Equipped with Chitosan-based Nanoreservoirs of Growth Factors. Macromol. Biosci. 2014, Jan;14(1):45-55.

I.A. KARPENKO, R. KREDER, C. VALENCIA, P. VILLA, C. MENDRE, B. MOUILLAC, Y. MELY, M. HIBERT, D. BONNET, & A.S. KLYMCHENKO. Red Fluorescent Turn-On Ligands for Imaging and Quantifying G Protein-Coupled Receptors in Live Cells. ChemBioChem. 2014, Feb 10;15(3):359-63

V.Y. POSTUPALENKO, O.M. ZAMOTAIEV, V.V SHVADCHAK, A.V. STRIZHAK, V.G. PIVOVARENKO, A.S. KLYMCHENKO, & Y. MELY. Dual-Fluorescence L-Amino Acid Reports Insertion and Orientation of Melittin Peptide in Cell Membranes. Bioconj. Chem. 2014, in press.

Darwich, Z., Klymchenko, A. S., Dujardin, D., and Mély, Y. (2014) Imaging lipid order changes in endosome membranes of live cells by using a Nile Red-based membrane probe, RSC Advances 4, 8481-8488.

de Rocquigny H., El Meshri S.E., Richert L., Didier P., Darlix J.L., & Mely Y. (2014). Role of the nucleocapsid region in HIV-1 Gag assembly as investigated by quantitative fluorescence-based microscopy. Virus Res. 193C, 78-88.

Deramaudt T.B., Dujardin D., Noulet F., Martin S., Vauchelles R., Takeda K. and Rondé P. (2014). Altering FAK-paxillin interactions reduces adhesion, migration and invasion processes. PLoS One 18;9(3):e92059.

Kolli-Bouhafs K., Sick E., Noulet F., Gies J.P., De Mey J. and Rondé P. (2014) “FAK competes for Src to promote migration against invasion in melanoma cells”. Cell Death Dis. 14;5:e1379

Beck C., Boehler C., Barbat J.G., Illuzzi G., Rondé P., Gauthier L.R., Magroun N., Lopez B.S., Scully R., Boussin F.D., Schreiber V and Dantzer F. (2014) “PARP3 affects the relative contribution of homologous recombination and non homologous end joining”. Nucleic Acids Res. 42(9):5616-3.


Kilin V., Glushonkov O., Herdly L., Klymchenko A., Richert L., Mely Y. (2015) Fluorescence Lifetime Imaging of Membrane Lipid Order with a ratiometric Fluorescent Probe, Biophysical Journal, 108, 2521-2531.

Kempf N., Didier P., Postupalenko V., Bucher B. & Mely Y. (2015) Investigation by high resolution microscopy of the internalization of neuropeptide Y bound to its Y1 receptor. Methods Appl. Fluoresc. 3, 025004,.

El Meshri S.E., Dujardin D., Godet J., Richert L., Boudier C., Darlix J.L., Didier P., Mely Y. & de Rocquigny H. (2015) Role of the nucleocapsid domain in HIV-1 Gag oligomerization and trafficking to the plasma membrane: A fluorescence lifetime imaging microscopy investigation. J. Mol. Biol. 427, 1480-1494.

Anton H., Taha N., Boutant E., Richert L., Khatter H., Klaholtz B., Rondé P., Real E., de Rocquigny H. and Mély Y. (2015) “Investigating the cellular distribution and interactions of HIV-1 Nucleocapsid protein by quantitative fluorescence microscopy” PloS One 10(2):e0116921.


Niko Y., Didier, P., Mély, Y., Konishi G.I. & Klymchenko A., 2016 (sous press) Bright and photostable push-pull pyrene dye visualizes lipid order variation between plasma and intracellular

Krifa M., El Meshri S, Bentouati N, Pizzi A, Sick E, Chekir-Ghedira L and Rondé P. (2016) “In vitro and in vivo anti-melanoma effects of Pituranthos tortuosus essential oil via inhibition of FAK and Src activities”. J. Cell. Biochem 117(5):1167-75.

Blandin AF, Noulet F, Renner G, Mercier MC, Choulier L, Vauchelles R, Rondé P, Carreiras F, Etienne-Selloum N, Vereb G, Lelong-Rebel I, Martin S, Dontenwill M, Lehmann M. (2016) Cancer Lett. 376(2):328-38.